Biochem/physiol Actions

Ileum contraction assay: negative

General description

Native, human des-Arg C3 complement component. Single chain, 76 amino acid peptide. Once the C3a peptide has been produced in human plasma or serum, it is rapidly converted to C3a des-Arg upon removal of the C-terminal arginine by endogenous serum carboxypeptidase N. This enzymatic process is considered to be a major mechanism for controlling C3a function in vivo because C3a des-Arg has ﹤1% of the biological activity expressed by the C3a peptide. Therefore, C3a des-Arg can serve as a negative control molecule in experiments involved with the biological activities of the intact C3a anaphylatoxin peptide.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kajita, T. and Hugli, T.E. 1991. Am. J. Pathol. 138, 1359.Bokish, V.A. and Muller-Eberhard, H.J. 1970. J. Clin. Invest.49, 2427.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, pH 7.2.

Preparation Note

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)